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    With.the.assistance.of.a.co-author,.this.popular.student.textbook.has.been.updated.to.include.techniques.such.as.membrane.chromatography,.aqueous.phase.partitioning,.engineering.recombinant.proteins.for.purification.and.due.to.the.rapid.advances.in.bioinformatics/proteomics,.a.discussion.of.the.analysis.of.complex.protein.mixtures.by.2D-electrophoresis.and.RPHPLC.prior.to.sequencing.by.mass.spectroscopyEasily...read...eBooks...on...smart...phones,...computers,...or...any...eBook...readers,...including...KindleView.on.ScienceDirect.Enzymes.2nd.Edition.Biochemistry,.Biotechnology,.Clinical.Chemistry.Authors:.T.Palmer.P.L.Bonner.eBook.ISBN:.9780857099921.Paperback.ISBN:.9781904275275.Imprint:.Woodhead.Publishing.Published.Date:.4th.April.2007.Page.Count:.432.Sorry,..we..arent..shipping..this..product..to..your..region..at..this..timeKey...Features...Provides...an...introduction...to...enzymology...and...a...balanced...account...of...the...theoretical...and...applied...aspects...of...the...subjectDiscusses...techniques...such...as...membrane...chromatography,...aqueous...phase...partitioning...and...engineering...recombinant...proteins...for...purificationIncludes...a...discussion...of...the...analysis...of...complex...protein...mixtures...by...2D-electrophoresis...and...RPHPLC...prior...to...sequencing...by...mass...spectroscopy...Readership...Undergraduate...students...Table...of...Contents...Authors...Preface...Part...1:...Structure...and...Function...of...Enzymes...1:...An...Introduction...to...Enzymes...1.1...WHAT...ARE...ENZYMES?...1.2...A...BRIEF...HISTORY...OF...ENZYMES...1.3...THE...NAMING...AND...CLASSIFICATION...OF...ENZYMES...SUMMARY...OF...CHAPTER...1...PROBLEMS...2:...The...Structure...of...Proteins...2.1...INTRODUCTION...2.2...AMINO...ACIDS,...THE...BUILDING...BLOCKS...OF...PROTEINS...2.3...THE...BASIS...OF...PROTEIN...STRUCTURE...2.4...THE...DETERMINATION...OF...PRIMARY...STRUCTURE...2.5...THE...DETERMINATION...OF...PROTEIN...STRUCTURE...BY...X-RAY...CRYSTALLOGRAPHY...2.6...THE...INVESTIGATION...OF...PROTEIN...STRUCTURE...IN...SOLUTION...SUMMARY...OF...CHAPTER...2...PROBLEMS...3:...The...Biosynthesis...and...Properties...of...Proteins...3.1...THE...BIOSYNTHESIS...OF...PROTEINS...3.2...THE...PROPERTIES...OF...PROTEINS...SUMMARY...OF...CHAPTER...3...PROBLEMS...4:...Specificity...of...Enzyme...Action...4.1...TYPES...OF...SPECIFICITY...4.2...THE...ACTIVE...SITE...4.3...THE...FISCHER...LOCK-AND-KEY...HYPOTHESIS...4.4...THE...KOSHLAND...INDUCED-FIT...HYPOTHESIS...4.5...HYPOTHESES...INVOLVING...STRAIN...OR...TRANSITION-STATE...STABILIZATION...4.6...FURTHER...COMMENTS...ON...SPECIFICITY...SUMMARY...OF...CHAPTER...4...5:...Monomeric...and...Oligomeric...Enzymes...5.1...MONOMERIC...ENZYMES...5.2...OLIGOMERIC...ENZYMES...SUMMARY...OF...CHAPTER...5...Part...2:...Kinetic...and...Chemical...Mechanisms...of...Enzyme-Catalysed...Reactions...6:...An...Introduction...to...Bioenergetics,...Catalysis...and...Kinetics...6.1...SOME...CONCEPTS...OF...BIOENERGETICS...6.2...FACTORS...AFFECTING...THE...RATES...OF...CHEMICAL...REACTIONS...6.3...KINETICS...OF...UNCATALYSED...CHEMICAL...REACTIONS...6.4...KINETICS...OF...ENZYME-CATALYSED...REACTIONS:...AN...HISTORICAL...INTRODUCTION...6.5...METHODS...USED...FOR...INVESTIGATING...THE...KINETICS...OF...ENZYME-CATALYSED...REACTIONS...6.6...THE...NATURE...OF...ENZYME...CATALYSIS...SUMMARY...OF...CHAPTER...6...PROBLEMS...7:...Kinetics...of...Single-Substrate...Enzyme-Catalysed...Reactions...7.1...THE...RELATIONSHIP...BETWEEN...INITIAL...VELOCITY...AND...SUBSTRATE...CONCENTRATION...7.2...RAPID-REACTION...kINETICS...7.3...THE...kING...AND...ALTMAN...PROCEDURE...SUMMARY...OF...CHAPTER...7...PROBLEMS...8:...Enzyme...Inhibition...8.1...INTRODUCTION...8.2...REVERSIBLE...INHIBITION...8.3...IRREVERSIBLE...INHIBITION...SUMMARY...OF...CHAPTER...8...PROBLEMS...9:...Kinetics...of...Multi-Substrate...Enzyme-Catalysed...Reactions...9.1...EXAMPLES...OF...POSSIBLE...MECHANISMS...9.2...STEADY-STATE...KINETICS...9.3...INVESTIGATION...OF...REACTION...MECHANISMS...USING...STEADY-STATE...METHODS...9.4...INVESTIGATION...OF...REACTION...MECHANISMS...USING...NON-STEADY-STATE...METHODS...SUMMARY...OF...CHAPTER...9...PROBLEMS...10:...The...Investigation...of...Active...Site...Structure...10.1...THE...IDENTIFICATION...OF...BINDING...SITES...AND...CATALYTIC...SITES...10.2...THE...INVESTIGATION...OF...THE...THREE-DIMENSIONAL...STRUCTURES...OF...ACTIVE...SITES...SUMMARY...OF...CHAPTER...10...PROBLEM...11:...The...chemical...nature...of...enzyme...catalysis...11.1...AN...INTRODUCTION...TO...REACTION...MECHANISMS...IN...ORGANIC...CHEMISTRY...11.2...MECHANISMS...OF...CATALYSIS...11.3...MECHANISMS...OF...REACTIONS...CATALYSED...BY...ENZYMES...WITHOUT...COFACTORS...11.4...METAL-ACTIVATED...ENZYMES...AND...METALLOENZYMES...11.5...THE...INVOLVEMENT...OF...COENZYMES...IN...ENZYME-CATALYSED...REACTIONS...SUMMARY...OF...CHAPTER...11...12:...The...Binding...of...Ligands...to...Proteins...12.1...INTRODUCTION...12.2...THE...BINDING...OF...A...LIGAND...TO...A...PROTEIN...HAVING...A...SINGLE...LIGAND-BINDING...SITE...12.3...COOPERATIVITY...12.4...POSITIVE...HOMOTROPIC...COOPERATIVITY...AND...THE...HILL...EQUATION...12.5...THE...ADAIR...EQUATION...FOR...THE...BINDING...OF...A...LIGAND...TO...A...PROTEIN...HAVING...TWO...BINDING...SITES...FOR...THAT...LIGAND...12.6...THE...ADAIR...EQUATION...FOR...THE...BINDING...OF...A...LIGAND...TO...A...PROTEIN...HAVING...THREE...BINDING...SITES...FOR...THAT...LIGAND...12.7...THE...ADAIR...EQUATION...FOR...THE...BINDING...OF...A...LIGAND...TO...A...PROTEIN...HAVING...FOUR...BINDING...SITES...FOR...THAT...LIGAND...12.8...INVESTIGATION...OF...COOPERATIVE...EFFECTS...12.9...THE...BINDING...OF...OXYGEN...TO...HAEMOGLOBIN...SUMMARY...OF...CHAPTER...12...PROBLEMS...13:...Sigmoidal...Kinetics...and...Allosteric...Enzymes...13.1...INTRODUCTION...13.2...THE...MONOD-WYMAN-CHANGEUX...(MWC)...MODEL...13.3...THE...KOSHLAND-NMETHY-FILMER...(KNF)...MODEL...13.4...DIFFERENTIATION...BETWEEN...MODELS...FOR...COOPERATIVE...BINDING...IN...PROTEINS...13.5...SIGMOIDAL...KINETICS...IN...THE...ABSENCE...OF...COOPERATIVE...BINDING...SUMMARY...OF...CHAPTER...13...PROBLEMS...14:...The...Significance...of...Sigmoidal...Behaviour...14.1...THE...PHYSIOLOGICAL...IMPORTANCE...OF...COOPERATIVE...OXYGEN-BINDING...BY...HEMOGLOBIN...14.2...ALLOSTERIC...ENZYMES...AND...METABOLIC...REGULATION...SUMMARY...OF...CHAPTER...14...Part...3:...Application...of...Enzymology...15:...Investigation...of...Enzymes...in...Biological...Preparations...15.1...CHOICE...OF...PREPARATION...FOR...THE...INVESTIGATION...OF...ENZYME...CHARACTERISTICS...15.2...ENZYME...ASSAY...15.3...INVESTIGATION...OF...SUB-CELLULAR...COMPARTMENTATION...OF...ENZYMES...SUMMARY...OF...CHAPTER...15...PROBLEM...16:...Extraction...and...Purification...of...Enzymes...16.1...EXTRACTION...OF...ENZYMES...16.2...PURIFICATION...OF...ENZYMES...16.3...DETERMINATION...OF...MOLECULAR...WEIGHTS...OF...ENZYMES...SUMMARY...OF...CHAPTER...16...PROBLEM...17:...Enzymes...as...Analytical...Reagents...17.1...THE...VALUE...OF...ENZYMES...AS...ANALYTICAL...REAGENTS...17.2...PRINCIPLES...OF...ENZYMATIC...ANALYSIS...17.3...HANDLING...ENZYMES...AND...COENZYMES...SUMMARY...OF...CHAPTER...17...PROBLEMS...18:...Instrumental...Techniques...Available...for...Use...in...Enzymatic...Analysis...18.1...PRINCIPLES...OF...THE...AVAILABLE...DETECTION...TECHNIQUES...18.2...AUTOMATION...IN...ENZYMATIC...ANALYSIS...18.3...HIGH-THROUGHPUT...ASSAYS...(HTA)...SUMMARY...OF...CHAPTER...18...19:...Applications...of...Enzymatic...Analysis...in...Medicine,...Forensic...Science...and...Industry...19.1...APPLICATIONS...IN...MEDICINE...19.2...APPLICATIONS...IN...FORENSIC...SCIENCE...19.3...APPLICATIONS...IN...INDUSTRY...SUMMARY...OF...CHAPTER...19...20:...Biotechnological...Applications...of...Enzymes...20.1...LARGE-SCALE...PRODUCTION...AND...PURIFICATION...OF...ENZYMES...20.2...IMMOBILIZED...ENZYMES...20.3...ENZYME...UTILIZATION...IN...INDUSTRY...SUMMARY...OF...CHAPTER...20...PROBLEMS...21:...Genomics,...Proteomics...and...Bioinformatics...21.1...ENZYMES...AND...RECOMBINANT...DNA...TECHNOLOGY...21.2...PROTEOMICS...21.3...ENZYMES...AND...BIOINFORMATICS...SUMMARY...OF...CHAPTER...21...PROBLEMS...Answers...to...Problems...Abbreviations...Index...Details...NoPriyanka...rated...it...really...liked...it...Aug...11,...2015...If..you..wish..to..place..a..tax..exempt..order..please..contact..usComputer..Science../..eBookEverything..curl15..Nov,..2017..eBook../..Geography../..Geolocation../..Geomatics../..Geospatial../..GISMapping..and..the..Citizen..Sensor14..Nov,..2017..Artificial..Intelligence../..Computer..Science../..eBookApplied..Artificial..Neural..Networks26..Oct,..2017..Artificial..Intelligence../..Computer..Science../..ProgrammingProgramming..Neural..Networks..with..Encog3..in..Java26..Oct,..2017..Computer..Science../..eBook../..ProgrammingJava..Based..Real..Time..Programming25..Oct,..2017 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